What I Do All Day

I heard that a few of you are disappointed that I don't talk about myself a little more often, so I figure I'll write a little post about how I'm doing and what my schoolwork is like. The short answer is that I'm doing great. I love being in school. I love it. I feel like this is what I was made for. At first I was really worried about coming back after being out of the game for three years; not just out of school, but not having even thought about science for those years. So I was relieved (and more than a little surprised) when everything came right back to me, as though I'd never left.
They definitely keep me busy around here. I'm only taking one class, but believe me, it's enough. Luckily for me, though, it's a really interesting class. Not only that, but after each lecture I feel like I'm just a little bit closer to the cutting edge of my field. It's a weird feeling. You go to school for years and years, and it seems like there's always more to learn, and now I'm getting to the point where people ask questions and the answer is, "We don't know yet." It's exciting to think that I'll be helping to push the boundaries of human knowledge.
On top of my class, I've been teaching a general chemistry lab class. It's similar to the TA work I did at Eastern, so I'm not too out of my element, but it's definitely more labor intensive. Aside from doing all the grading, I have to actually keep a grade book, prepare short (really short) lectures, and even discipline the class if necessary (I've only had to do this once, thankfully). I'm like a real teacher now; some of them even call me Mr. Lowry, although I try and get them to call me Josh. There is still a bit of a difference, though; I doubt too many high school teachers have students asking for their phone numbers.
And of course there's my research, which is the really exciting part. In fact, I can't wait until I no longer have to take classes so I can spend my time doing my research. I've been working on a few different projects since I showed up this summer, and one of them has yielded some exciting results. What we do is something called RNAi, where we feed our worms this special food that targets one of their genes and shuts it off. Then, we look at one-celled embryos, watch them divide, and see if there's any difference between our mutant and a normal cell. Most of the time there's no difference; I mean, worms have ~30,000 genes, and we're shutting off one. But every once in a while, you get something exciting, and that's what happened to me. Here are some videos I made of this first cell division:



In this one, the DNA and the centrosomes (no, I don't expect you to know what they are) are labeled with a special protein that glows green when you shine a laser on it.


This one shows microtubles labeled in red. These both show the same mutant, just with different cell parts visible. I know they're not in color, but when I look at them under the microscope those are the colors I see. When we process them, they lose their color. Still, it's pretty cool to be able to watch all this happening right in front of you. The first cell division actually takes between 10-15mins, so this is time-lapse video you're watching, but still, this is an actual cell going through an actual division. I think it's cool every time I watch it.

So yeah, that's what I've been doing. It keeps me busy, but I'm loving every minute. And with that, it's back to work for me.